Itou seeks to subvert and split the Shinsengumi, especially after he finds out Yamanami is still alive. Chizuru still hides her quick-healing ability when she is injured by an ochimizu-maddened soldier. Osen and her kunoichi Kimigiku come to collect Chizuru from the Shinsengumi headquarters for her own safety; Osen claims an ancient oni bloodline and forces Chizuru to admit her own oni status. The Rasetsu Squad are activated when Kazura attempts yet again to kidnap Chizuru from the headquarters.

• Osamu Yamasaki
• Tsunekiyo Fujisawa

• Shin'ichirou Miki
• Houko Kuwashima
• Shin-ichiro Miki